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KMID : 1094720110160040821
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 4 p.821 ~ p.821
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Oxidation and removal of industrial textile dyes by a novel peroxidase extracted from post-harvest lentil (Lens culinaris L.) stubble
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Hidalgo Nazaret
Mangiameli Giulia
Manzano Teresa
Zhadan Galina G.
Kennedy John F.
Shnyrov Valery L.
Roig Manuel G.
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Abstract
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The degradation and removal of a series of dyes used in the textile industry for polyester/wool (PES/WO) blends and present in effluents, such as Green, Ash-Grey, Black, Navy Blue, Red and Yellow Domalan, and Orange and Red Bemacid, by catalytic action, in the presence of H2O2, of extracts of a novel peroxidase from postharvest lentil stubble was investigated. The extracts of this peroxidase (LSP) were effective in degrading these lastgeneration textile dyes, especially Green Domalan, Orange Bemacid, Grey and Black Domalan. A sensitivity study was carried out for Green Domalan biodegradation to determine the effects of process parameters such as pH, H2O2, enzyme and dye concentrations, contact and centrifugation times, and temperature. Standard ecotoxicity studies performed with Vibrio fischeri revealed that the dye solutions treated with peroxidase and H2O2 were less ecotoxic than the untreated ones.
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KEYWORD
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azo, anthraquinone, textile, dyes, peroxidase, oxidation, decolorization, ecotoxicity test
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